50X TAE Buffer, 100 mL
JPY 7286
Price Details
Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )
*All items will import from アメリカ
Ubuyはお客様のセキュリティとプライバシーの保護に努めています。当社の高度な決済セキュリティシステムは、AES(高度暗号化標準)およびSSL(セキュアソケットレイヤー)プロトコルを使用して送信中の情報を暗号化することで機密性を確保しています。お客様の決済情報は第三者の販売者と共有されることはなく、100%安全に保護されています。
50X TAE Buffer protects DNA during electrophoresis, ensuring your results are reliable and accurate.
Fast
Shipping
Free
Return*
安全な梱包
100%本物
PCI DSS 準拠
ISO 27001 保証
製品詳細
| Item Weight | 0.25 lbs (110 grams) |
製品説明書
50X TAE Buffer, 100 mL
顧客の質問と回答
-
質問:
What is 50X TAE Buffer used for?
答え: 50X TAE Buffer is primarily used in molecular biology for the electrophoresis of nucleic acids. This buffer maintains a stable pH during the separation of DNA and RNA and provides essential ions that help in the migration of nucleic acids through gels. When diluted to 1X concentration, it is optimal for running agarose gels, making it crucial in applications such as gel electrophoresis and DNA fragment analysis. -
質問:
How do I dilute 50X TAE Buffer for use?
答え: To use 50X TAE Buffer, it needs to be diluted to 1X concentration before application. This is achieved by mixing 1 part of the 50X solution with 49 parts of distilled water. For example, if you need 100 mL of 1X TAE Buffer, combine 2 mL of 50X TAE Buffer with 98 mL of water. This dilution is vital for effective DNA separation and band visualization in gel electrophoresis. -
質問:
What is the shelf life of 50X TAE Buffer?
答え: The shelf life of 50X TAE Buffer varies depending on storage conditions, but generally, it can remain stable for extended periods if kept in a cool and dry place. To ensure optimal performance, always check for any turbidity, discoloration, or precipitation before use. Proper storage will help maintain its effectiveness for experiments that involve DNA manipulation and analysis, reducing the risk of experimental failure. -
質問:
Is 50X TAE Buffer compatible with agarose gel electrophoresis?
答え: Yes, 50X TAE Buffer is highly compatible with agarose gel electrophoresis. It provides the necessary ionic environment that stabilizes the DNA while separating it based on size. This buffer is preferred for applications requiring high resolution in DNA fragment size determination. When diluted appropriately to 1X, it ensures effective migration of DNA through the gel matrix, allowing for clear visualization of the bands. -
質問:
Can I use 50X TAE Buffer for RNA electrophoresis?
答え: Yes, 50X TAE Buffer can be used for RNA electrophoresis, but its use in RNA analysis should be approached with care. It is important to ensure that the RNA samples are not degraded, and the buffer is fresh. RNA is sensitive to degradation; therefore, using 50X TAE Buffer allows better resolution of RNA bands when diluted correctly, promoting accurate electrophoresis results. -
質問:
What are the components of 50X TAE Buffer?
答え: 50X TAE Buffer consists of Tris base, acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent to maintain pH, acetic acid provides the acidic environment, and EDTA chelates divalent metal ions, which can otherwise interfere with DNA and enzymes. The combination creates a stable buffer system essential for nucleic acid studies, providing researchers with optimal conditions for their experiments. -
質問:
Is 50X TAE Buffer necessary for DNA gel extraction?
答え: 50X TAE Buffer is not strictly necessary for DNA gel extraction but is useful in maintaining the integrity of DNA post-electrophoresis. After running your gel, the buffer can help rehydrate the gel and provide a stable pH environment for further DNA processing. It can also be beneficial when preparing samples for downstream applications, allowing for better recovery of DNA fragments due to its compatibility with various procedures. -
質問:
How should I store 50X TAE Buffer?
答え: To preserve the stability of 50X TAE Buffer, it should be stored in a cool, dark place at room temperature or refrigerated. Make sure the container is sealed tightly to prevent contamination. Proper storage conditions help prevent degradation or changes in composition, ensuring reliable results during experiments involving nucleic acids, such as PCR preparation or gel electrophoresis. -
質問:
What are some common applications for using 50X TAE Buffer?
答え: 50X TAE Buffer has several applications, primarily in molecular biology, including gel electrophoresis for DNA and RNA, restriction enzyme digestion, and PCR preparations. It helps facilitate the migration of nucleic acids through gels, aiding in the quantification and analysis of DNA fragments. Researchers often use it for cloning experiments and during the preparation of samples for sequencing, ensuring high-quality results. -
質問:
Where can I buy 50X TAE Buffer, 100 mL?
答え: You can buy 50X TAE Buffer, 100 mL on Ubuy in Japan. Ubuy provides a convenient online shopping platform where you can find various laboratory supplies, including different concentrations of TAE Buffer, ensuring you get the right product for your research needs.
eCon Lab Supply Buffers Editorial Review
お客様のレビュー&評価
-
5 星
100%
-
4 星
0%
-
3 星
0%
-
2 星
0%
-
1 星
0%
この商品のレビュー
お考えをお聞かせください
長所
- High purity formula
- Convenient 100 mL size
- Well-suited for various applications
- Easy to store and use
- Reliable performance
短所
- Cap can be slightly hard to open.
Product Price History
重要な情報
- 注意:国際運送の商品に関して、製造会社保障は無効になる可能性、アフターサービスは受けれない可能性、取説や安全情報は発送先の言語になっていない可能性ある。商品とその付属品は配送先の国の規格、仕様、ラベル表示法などに適応していない可能性があります。また、配送先の国の電力企画に適応しいない(アダプタや変換器を必要とする)可能性があります。ご注文の商品は配送先の国に輸入することは合法なのかを確認するのは購入者の責任になります。Ubuyからご購入の際、受け取り者は正式な輸入者となり、配送先の全ての法律やルールに遵守する必要があります。
- Ubuyはグローバル検索エンジンのためリストにある全ての商品が購入できないことがあります。商品は輸出規制、貿易規制があります。
JPY 7286
今すぐ注文すると 頃に届きます 水曜日, 7月 08
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Ubuyはお客様のセキュリティとプライバシーの保護に努めています。当社の高度な決済セキュリティシステムは、AES(高度暗号化標準)およびSSL(セキュアソケットレイヤー)プロトコルを使用して送信中の情報を暗号化することで機密性を確保しています。お客様の決済情報は第三者の販売者と共有されることはなく、100%安全に保護されています。
特徴と利点
- Contains 2M Tris, 1M Acetic acid, and 0.05M EDTA for effective nucleic acid electrophoresis.
- Inhibits nucleases to protect DNA integrity during experiments.
- Powers efficient running of both genomic and supercoiled DNA.
- Faster dsDNA run compared to TBE buffer, enhancing experiment efficiency.
- Manufactured under strict quality control in an ISO certified facility.
- Ideal for research purposes, compliant with Good Manufacturing Practices.